Marko Horb

Senior Scientist and NXR Director

Marko Horb Headshot
Contact Information
Education
Ph.D., Cellular and Developmental Biology, Stony Brook University, 1998
B.Sc., Cell and Structural Biology, University of Illinois at Urbana Champaign, 1993
小蓝视频 Affiliation
Bell Center
Research Area
Cell Biology
Developmental Biology
Models of Human Disease
Regeneration
Lab Website
Horb Lab

The Horb lab is focused on generating聽Xenopus聽models of human disease using CRISPR-Cas. We work closely with聽Xenopus聽and non-Xenopus聽researchers to generate genetic knockouts of specific genes. As part of this project, visiting scientists can come for short- or long-term visits and work in the NXR with its staff on these various mutants. To date, we have generated over 175 mutants either in聽X. tropicalis听辞谤听X. laevis. In addition, Dr. Horb is the Director of the National Xenopus Resource (NXR), which is the national stock center for聽X. laevis听补苍诲听X. tropicalis.聽The NXR is funded by an award from the National Institute of Health, Office of the Director, Office of Research Infrastructure Programs (ORIP) and from the Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD). The NXR provides a facility for breeding聽X. laevis聽and聽X. tropicalis, maintaining genetic stocks and providing stocks to researchers. The NXR also develops new experimental tools and husbandry techniques for the聽Xenopus聽research community. Lastly, the NXR serves as a training center for advanced technologies (e.g. husbandry, cell biology, imaging, genetics, transgenesis, genomics) through workshops and meetings.

News and Research Highlights
Selected Publications

Coppenrath K, Tavares A, Shaidani N, Wlizla M, Moody SA,聽Horb M. (2021) Generation of a new聽six1-null line in聽Xenopus tropicalis聽for study of development and congenital disease. Genesis e23453

Horb M, Wlizla M, Abu-Daya A, McNamara S, Gajdasik D, Igawa T, Suzuki A, Ogino H, Noble A; Centre de Ressource Biologique Xenope team in France, Robert J, James-Zorn C, Guille M. (2019)聽Xenopus聽resources: Transgenic, inbred, and mutant animals, training opportunities, and web-based support. Front Physiol. Apr 25;10:387. eCollection 2019. Review. PMCID: PMC6497014

McNamara S., Wlizla M.,聽Horb M.E.聽(2018) Husbandry, General Care, and Transportation of聽Xenopus laevis听补苍诲听Xenopus tropicalis. Methods Mol Biol. 1865:1-17. PMCID: PMC6421069

Corkins, M.E., Hanania, H.L., Krneta-Stankic, V., DeLay, B.D., Pearl, E.J., Lee, M., Ji, H., Davidson, A.J.,聽Horb, M.E.聽and Miller, R.K. (2018) Transgenic聽Xenopus laevis聽line for in vivo labeling of nephrons within the kidney.聽Genes聽9, 197. (PMCID: PMC5924539)

Pearl E., Morrow S., Noble A., Lerebours A.,聽Horb M., Guille M. (2017) An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs. Theriogenology. 92, 149-155. (PMCID: PMC5340284)

Ratzan, W., Falco, R., Salanga, C., Salanga, M. and聽Horb, M.E.聽(2017). Generation of a聽Xenopus laevis聽F1 albino J strain by genome editing and oocyte host transfer. Developmental Biology 426, 188-193 (PMC5025372)